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c6 cat atcc ccl 107  (ATCC)


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    ATCC c6 cat atcc ccl 107
    C6 Cat Atcc Ccl 107, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c6+atcc+cat/C6/10__1021_slash_acsphotonics__5c01071-236-0-3
    Average 96 stars, based on 1959 article reviews
    c6 cat atcc ccl 107 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Hydrophilic Interaction Liquid Chromatography:

    Article Title: Identification of the Nicotinamide Salvage Pathway as a New Toxification Route for Antimetabolites.
    Article Snippet: Cat#354234 NAD Sigma-Aldrich Cat#N3014 NADH Sigma-Aldrich Cat#N8129 ATP Sigma-Aldrich Cat#A2383 GLUCOSE [U-14C] Perkin Elmer Cat#NEC042V250UC TMRE Sigma-Aldrich Cat#87917 FCCP Sigma-Aldrich Cat#C2920 Nicotinamide Mononucleotide (NMN) Sigma-Aldrich Cat#N3501 Nicotinamide Riboside (NR) ChromaDex Cat#ASB-00014314 Nicotinamide (NAM) Sigma-Aldrich Cat#72340 5-Phospho-D-ribose 1-diphosphate (PRPP) Sigma-Aldrich Cat#P8296 Inosine 5’-monophosphate Sigma-Aldrich Cat#57510 DL-Glyceraldehyde 3’-phosphate Sigma-Aldrich Cat#G5251 Trizol Reagent ThermoFisher Cat#15596026 iScript BioRad Cat#1708890 SYBR Green PCR kit BioRad Cat#1725271 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A7906 Bovine Serum Albumin (BSA for cycling assay) Sigma-Aldrich Cat#A8531-1VL Riboflavin 50-monophosphate (FMN) Sigma-Aldrich Cat#F8399 Resazurin Sigma-Aldrich Cat#R7017 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma-Aldrich Cat#I6758 Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#75259 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy1-propanesulfonate (CHAPSO) Sigma-Aldrich Cat#C3649 DL-Dithiothreitol Sigma-Aldrich Cat#43819 Phenylmethanesulfonyl fluoride (PMSF) Fluka Cat#78830 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8849 Ampicillin Sigma-Aldrich Cat#A9518 Kanamycin Sigma-Aldrich Cat#K4000 HisTrap HP column GE Healthcare Cat#17-5247-01 PD-10 desalting columns (Sephadex G-25) GE Healthcare Cat#17-0851-01 PD MiniTrap (Sephadex )G-25 GE Healthcare Cat#28-9180-07 Supelcosil LC-18-S HPLC column Supelco (Sigma-Aldrich) Cat#58928-U Supelcosil LC-18-T HPLC column Supelco (Sigma-Aldrich) Cat#58971 (Continued on next page) Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Kinetex 2.6 mm C18 100A Phenomenex Cat#00F-4462-E0 Kinetex 2.6 mm HILIC 100A Phenomenex Cat#00F-4461-E0 TRACER EXTRASIL ODS2 5 mm Phenomenex Cat#N34544 Glyceraldehyde-3-phosphate Dehydrogenase Sigma-Aldrich Cat#G9263 Inosine Monophosphate Dehydrogenase Type II human Sigma-Aldrich Cat#I1782 Diaphorase from Clostridium kluyveri Sigma-Aldrich Cat#D5540 pET15b vector Novagen, EMD Millipore Cat#69661-3 Recombinant Human NAMPT This study N/A pTrcHisA- NMNAT1 Sorci et al., 2007 N/A pET28c- NMNAT2 Brunetti et al., 2010 N/A pTrcHisA- NMNAT3 Sorci et al., 2007 N/A Recombinant E.coli NMN deamidase (PncC) Zamporlini et al., 2014 N/A Recombinant B. anthracis NaMN adenylyltransferase (NadD) Zamporlini et al., 2014 N/A Recombinant B. anthracis NAD synthetase (NadE) Zamporlini et al., 2014 N/A a-cyano-hydroxycynnamic acid Sigma-Aldrich Cat#C2020 Aniline Sigma-Aldrich Cat#51788 9-aminoacridine Sigma-Aldrich Cat#92817 Oligonucleotides Oligonucleotides used, see Table S1 This study N/A Bacterial strains One Shot BL21 Star (DE3) Chemically Competent E.coli cells Invitrogen Cat#440049 One Shot TOP 10 Chemically Competent E.coli cells Invitrogen Cat#404003 Critical Commercial Assays ATPlite Luminescence Assay Perkin Elmer Cat#6016943 Bradford Protein Assay BioRad Cat#5000201 Lactate assay kit BioVision Cat#K607 Human NMNAT2 This study UniProtKB/Swiss-Prot: Q9BZQ4.1 Experimental Models: Cell Lines SH-SY5Y ATCC Cat#CRL-2266 HeLa ATCC Cat#CCL-2 INS-1 ATCC Cat#CRL-2057 SK-Hep-1 ATCC CatHTB-52 C6 ATCC Cat#CCL-107 NCI-H295R ATCC Cat#CRL-2128 CAPAN-1 ATCC Cat#HTB-79 JURKAT ATCC Cat#TIB-152 MeWo ATCC Cat#HTB-65 A375 ATCC Cat#CRL-1619 SK-Mel-2 CRL-ITT, Pisa N/A SK-Mel-5 CRL-ITT, Pisa N/A SK-Mel-28 CRL-ITT, Pisa N/A M26C CRL-ITT, Florence N/A M33x CRL-ITT, Florence N/A U937 ATCC Cat#CRL-1593 Experimental Models: Organisms/Strains Athymic nude mice (Foxn1 nu/nu) Envigo N/A (Continued on next page) e2 Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms VegaZZ Drug Design Laboratory http://www.vegazz.net I-TASSER Zhang Lab http://zhanglab.ccmb.med.umich.edu/ I-TASSER Verify_3D UCLA- DOE LAB http://services.mbi.ucla.edu/Verify_3D/ Thermo ImageQuest ThermoFisher Cat#10137985 GraphPad Prism version 7.00 for Windows GraphPad Software https://www.graphpad.com/

    Recombinant:

    Article Title: Identification of the Nicotinamide Salvage Pathway as a New Toxification Route for Antimetabolites.
    Article Snippet: Cat#354234 NAD Sigma-Aldrich Cat#N3014 NADH Sigma-Aldrich Cat#N8129 ATP Sigma-Aldrich Cat#A2383 GLUCOSE [U-14C] Perkin Elmer Cat#NEC042V250UC TMRE Sigma-Aldrich Cat#87917 FCCP Sigma-Aldrich Cat#C2920 Nicotinamide Mononucleotide (NMN) Sigma-Aldrich Cat#N3501 Nicotinamide Riboside (NR) ChromaDex Cat#ASB-00014314 Nicotinamide (NAM) Sigma-Aldrich Cat#72340 5-Phospho-D-ribose 1-diphosphate (PRPP) Sigma-Aldrich Cat#P8296 Inosine 5’-monophosphate Sigma-Aldrich Cat#57510 DL-Glyceraldehyde 3’-phosphate Sigma-Aldrich Cat#G5251 Trizol Reagent ThermoFisher Cat#15596026 iScript BioRad Cat#1708890 SYBR Green PCR kit BioRad Cat#1725271 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A7906 Bovine Serum Albumin (BSA for cycling assay) Sigma-Aldrich Cat#A8531-1VL Riboflavin 50-monophosphate (FMN) Sigma-Aldrich Cat#F8399 Resazurin Sigma-Aldrich Cat#R7017 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma-Aldrich Cat#I6758 Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#75259 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy1-propanesulfonate (CHAPSO) Sigma-Aldrich Cat#C3649 DL-Dithiothreitol Sigma-Aldrich Cat#43819 Phenylmethanesulfonyl fluoride (PMSF) Fluka Cat#78830 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8849 Ampicillin Sigma-Aldrich Cat#A9518 Kanamycin Sigma-Aldrich Cat#K4000 HisTrap HP column GE Healthcare Cat#17-5247-01 PD-10 desalting columns (Sephadex G-25) GE Healthcare Cat#17-0851-01 PD MiniTrap (Sephadex )G-25 GE Healthcare Cat#28-9180-07 Supelcosil LC-18-S HPLC column Supelco (Sigma-Aldrich) Cat#58928-U Supelcosil LC-18-T HPLC column Supelco (Sigma-Aldrich) Cat#58971 (Continued on next page) Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Kinetex 2.6 mm C18 100A Phenomenex Cat#00F-4462-E0 Kinetex 2.6 mm HILIC 100A Phenomenex Cat#00F-4461-E0 TRACER EXTRASIL ODS2 5 mm Phenomenex Cat#N34544 Glyceraldehyde-3-phosphate Dehydrogenase Sigma-Aldrich Cat#G9263 Inosine Monophosphate Dehydrogenase Type II human Sigma-Aldrich Cat#I1782 Diaphorase from Clostridium kluyveri Sigma-Aldrich Cat#D5540 pET15b vector Novagen, EMD Millipore Cat#69661-3 Recombinant Human NAMPT This study N/A pTrcHisA- NMNAT1 Sorci et al., 2007 N/A pET28c- NMNAT2 Brunetti et al., 2010 N/A pTrcHisA- NMNAT3 Sorci et al., 2007 N/A Recombinant E.coli NMN deamidase (PncC) Zamporlini et al., 2014 N/A Recombinant B. anthracis NaMN adenylyltransferase (NadD) Zamporlini et al., 2014 N/A Recombinant B. anthracis NAD synthetase (NadE) Zamporlini et al., 2014 N/A a-cyano-hydroxycynnamic acid Sigma-Aldrich Cat#C2020 Aniline Sigma-Aldrich Cat#51788 9-aminoacridine Sigma-Aldrich Cat#92817 Oligonucleotides Oligonucleotides used, see Table S1 This study N/A Bacterial strains One Shot BL21 Star (DE3) Chemically Competent E.coli cells Invitrogen Cat#440049 One Shot TOP 10 Chemically Competent E.coli cells Invitrogen Cat#404003 Critical Commercial Assays ATPlite Luminescence Assay Perkin Elmer Cat#6016943 Bradford Protein Assay BioRad Cat#5000201 Lactate assay kit BioVision Cat#K607 Human NMNAT2 This study UniProtKB/Swiss-Prot: Q9BZQ4.1 Experimental Models: Cell Lines SH-SY5Y ATCC Cat#CRL-2266 HeLa ATCC Cat#CCL-2 INS-1 ATCC Cat#CRL-2057 SK-Hep-1 ATCC CatHTB-52 C6 ATCC Cat#CCL-107 NCI-H295R ATCC Cat#CRL-2128 CAPAN-1 ATCC Cat#HTB-79 JURKAT ATCC Cat#TIB-152 MeWo ATCC Cat#HTB-65 A375 ATCC Cat#CRL-1619 SK-Mel-2 CRL-ITT, Pisa N/A SK-Mel-5 CRL-ITT, Pisa N/A SK-Mel-28 CRL-ITT, Pisa N/A M26C CRL-ITT, Florence N/A M33x CRL-ITT, Florence N/A U937 ATCC Cat#CRL-1593 Experimental Models: Organisms/Strains Athymic nude mice (Foxn1 nu/nu) Envigo N/A (Continued on next page) e2 Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms VegaZZ Drug Design Laboratory http://www.vegazz.net I-TASSER Zhang Lab http://zhanglab.ccmb.med.umich.edu/ I-TASSER Verify_3D UCLA- DOE LAB http://services.mbi.ucla.edu/Verify_3D/ Thermo ImageQuest ThermoFisher Cat#10137985 GraphPad Prism version 7.00 for Windows GraphPad Software https://www.graphpad.com/

    Luminescence Assay:

    Article Title: Identification of the Nicotinamide Salvage Pathway as a New Toxification Route for Antimetabolites.
    Article Snippet: Cat#354234 NAD Sigma-Aldrich Cat#N3014 NADH Sigma-Aldrich Cat#N8129 ATP Sigma-Aldrich Cat#A2383 GLUCOSE [U-14C] Perkin Elmer Cat#NEC042V250UC TMRE Sigma-Aldrich Cat#87917 FCCP Sigma-Aldrich Cat#C2920 Nicotinamide Mononucleotide (NMN) Sigma-Aldrich Cat#N3501 Nicotinamide Riboside (NR) ChromaDex Cat#ASB-00014314 Nicotinamide (NAM) Sigma-Aldrich Cat#72340 5-Phospho-D-ribose 1-diphosphate (PRPP) Sigma-Aldrich Cat#P8296 Inosine 5’-monophosphate Sigma-Aldrich Cat#57510 DL-Glyceraldehyde 3’-phosphate Sigma-Aldrich Cat#G5251 Trizol Reagent ThermoFisher Cat#15596026 iScript BioRad Cat#1708890 SYBR Green PCR kit BioRad Cat#1725271 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A7906 Bovine Serum Albumin (BSA for cycling assay) Sigma-Aldrich Cat#A8531-1VL Riboflavin 50-monophosphate (FMN) Sigma-Aldrich Cat#F8399 Resazurin Sigma-Aldrich Cat#R7017 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma-Aldrich Cat#I6758 Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#75259 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy1-propanesulfonate (CHAPSO) Sigma-Aldrich Cat#C3649 DL-Dithiothreitol Sigma-Aldrich Cat#43819 Phenylmethanesulfonyl fluoride (PMSF) Fluka Cat#78830 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8849 Ampicillin Sigma-Aldrich Cat#A9518 Kanamycin Sigma-Aldrich Cat#K4000 HisTrap HP column GE Healthcare Cat#17-5247-01 PD-10 desalting columns (Sephadex G-25) GE Healthcare Cat#17-0851-01 PD MiniTrap (Sephadex )G-25 GE Healthcare Cat#28-9180-07 Supelcosil LC-18-S HPLC column Supelco (Sigma-Aldrich) Cat#58928-U Supelcosil LC-18-T HPLC column Supelco (Sigma-Aldrich) Cat#58971 (Continued on next page) Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Kinetex 2.6 mm C18 100A Phenomenex Cat#00F-4462-E0 Kinetex 2.6 mm HILIC 100A Phenomenex Cat#00F-4461-E0 TRACER EXTRASIL ODS2 5 mm Phenomenex Cat#N34544 Glyceraldehyde-3-phosphate Dehydrogenase Sigma-Aldrich Cat#G9263 Inosine Monophosphate Dehydrogenase Type II human Sigma-Aldrich Cat#I1782 Diaphorase from Clostridium kluyveri Sigma-Aldrich Cat#D5540 pET15b vector Novagen, EMD Millipore Cat#69661-3 Recombinant Human NAMPT This study N/A pTrcHisA- NMNAT1 Sorci et al., 2007 N/A pET28c- NMNAT2 Brunetti et al., 2010 N/A pTrcHisA- NMNAT3 Sorci et al., 2007 N/A Recombinant E.coli NMN deamidase (PncC) Zamporlini et al., 2014 N/A Recombinant B. anthracis NaMN adenylyltransferase (NadD) Zamporlini et al., 2014 N/A Recombinant B. anthracis NAD synthetase (NadE) Zamporlini et al., 2014 N/A a-cyano-hydroxycynnamic acid Sigma-Aldrich Cat#C2020 Aniline Sigma-Aldrich Cat#51788 9-aminoacridine Sigma-Aldrich Cat#92817 Oligonucleotides Oligonucleotides used, see Table S1 This study N/A Bacterial strains One Shot BL21 Star (DE3) Chemically Competent E.coli cells Invitrogen Cat#440049 One Shot TOP 10 Chemically Competent E.coli cells Invitrogen Cat#404003 Critical Commercial Assays ATPlite Luminescence Assay Perkin Elmer Cat#6016943 Bradford Protein Assay BioRad Cat#5000201 Lactate assay kit BioVision Cat#K607 Human NMNAT2 This study UniProtKB/Swiss-Prot: Q9BZQ4.1 Experimental Models: Cell Lines SH-SY5Y ATCC Cat#CRL-2266 HeLa ATCC Cat#CCL-2 INS-1 ATCC Cat#CRL-2057 SK-Hep-1 ATCC CatHTB-52 C6 ATCC Cat#CCL-107 NCI-H295R ATCC Cat#CRL-2128 CAPAN-1 ATCC Cat#HTB-79 JURKAT ATCC Cat#TIB-152 MeWo ATCC Cat#HTB-65 A375 ATCC Cat#CRL-1619 SK-Mel-2 CRL-ITT, Pisa N/A SK-Mel-5 CRL-ITT, Pisa N/A SK-Mel-28 CRL-ITT, Pisa N/A M26C CRL-ITT, Florence N/A M33x CRL-ITT, Florence N/A U937 ATCC Cat#CRL-1593 Experimental Models: Organisms/Strains Athymic nude mice (Foxn1 nu/nu) Envigo N/A (Continued on next page) e2 Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms VegaZZ Drug Design Laboratory http://www.vegazz.net I-TASSER Zhang Lab http://zhanglab.ccmb.med.umich.edu/ I-TASSER Verify_3D UCLA- DOE LAB http://services.mbi.ucla.edu/Verify_3D/ Thermo ImageQuest ThermoFisher Cat#10137985 GraphPad Prism version 7.00 for Windows GraphPad Software https://www.graphpad.com/

    Bradford Protein Assay:

    Article Title: Identification of the Nicotinamide Salvage Pathway as a New Toxification Route for Antimetabolites.
    Article Snippet: Cat#354234 NAD Sigma-Aldrich Cat#N3014 NADH Sigma-Aldrich Cat#N8129 ATP Sigma-Aldrich Cat#A2383 GLUCOSE [U-14C] Perkin Elmer Cat#NEC042V250UC TMRE Sigma-Aldrich Cat#87917 FCCP Sigma-Aldrich Cat#C2920 Nicotinamide Mononucleotide (NMN) Sigma-Aldrich Cat#N3501 Nicotinamide Riboside (NR) ChromaDex Cat#ASB-00014314 Nicotinamide (NAM) Sigma-Aldrich Cat#72340 5-Phospho-D-ribose 1-diphosphate (PRPP) Sigma-Aldrich Cat#P8296 Inosine 5’-monophosphate Sigma-Aldrich Cat#57510 DL-Glyceraldehyde 3’-phosphate Sigma-Aldrich Cat#G5251 Trizol Reagent ThermoFisher Cat#15596026 iScript BioRad Cat#1708890 SYBR Green PCR kit BioRad Cat#1725271 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A7906 Bovine Serum Albumin (BSA for cycling assay) Sigma-Aldrich Cat#A8531-1VL Riboflavin 50-monophosphate (FMN) Sigma-Aldrich Cat#F8399 Resazurin Sigma-Aldrich Cat#R7017 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma-Aldrich Cat#I6758 Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#75259 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy1-propanesulfonate (CHAPSO) Sigma-Aldrich Cat#C3649 DL-Dithiothreitol Sigma-Aldrich Cat#43819 Phenylmethanesulfonyl fluoride (PMSF) Fluka Cat#78830 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8849 Ampicillin Sigma-Aldrich Cat#A9518 Kanamycin Sigma-Aldrich Cat#K4000 HisTrap HP column GE Healthcare Cat#17-5247-01 PD-10 desalting columns (Sephadex G-25) GE Healthcare Cat#17-0851-01 PD MiniTrap (Sephadex )G-25 GE Healthcare Cat#28-9180-07 Supelcosil LC-18-S HPLC column Supelco (Sigma-Aldrich) Cat#58928-U Supelcosil LC-18-T HPLC column Supelco (Sigma-Aldrich) Cat#58971 (Continued on next page) Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Kinetex 2.6 mm C18 100A Phenomenex Cat#00F-4462-E0 Kinetex 2.6 mm HILIC 100A Phenomenex Cat#00F-4461-E0 TRACER EXTRASIL ODS2 5 mm Phenomenex Cat#N34544 Glyceraldehyde-3-phosphate Dehydrogenase Sigma-Aldrich Cat#G9263 Inosine Monophosphate Dehydrogenase Type II human Sigma-Aldrich Cat#I1782 Diaphorase from Clostridium kluyveri Sigma-Aldrich Cat#D5540 pET15b vector Novagen, EMD Millipore Cat#69661-3 Recombinant Human NAMPT This study N/A pTrcHisA- NMNAT1 Sorci et al., 2007 N/A pET28c- NMNAT2 Brunetti et al., 2010 N/A pTrcHisA- NMNAT3 Sorci et al., 2007 N/A Recombinant E.coli NMN deamidase (PncC) Zamporlini et al., 2014 N/A Recombinant B. anthracis NaMN adenylyltransferase (NadD) Zamporlini et al., 2014 N/A Recombinant B. anthracis NAD synthetase (NadE) Zamporlini et al., 2014 N/A a-cyano-hydroxycynnamic acid Sigma-Aldrich Cat#C2020 Aniline Sigma-Aldrich Cat#51788 9-aminoacridine Sigma-Aldrich Cat#92817 Oligonucleotides Oligonucleotides used, see Table S1 This study N/A Bacterial strains One Shot BL21 Star (DE3) Chemically Competent E.coli cells Invitrogen Cat#440049 One Shot TOP 10 Chemically Competent E.coli cells Invitrogen Cat#404003 Critical Commercial Assays ATPlite Luminescence Assay Perkin Elmer Cat#6016943 Bradford Protein Assay BioRad Cat#5000201 Lactate assay kit BioVision Cat#K607 Human NMNAT2 This study UniProtKB/Swiss-Prot: Q9BZQ4.1 Experimental Models: Cell Lines SH-SY5Y ATCC Cat#CRL-2266 HeLa ATCC Cat#CCL-2 INS-1 ATCC Cat#CRL-2057 SK-Hep-1 ATCC CatHTB-52 C6 ATCC Cat#CCL-107 NCI-H295R ATCC Cat#CRL-2128 CAPAN-1 ATCC Cat#HTB-79 JURKAT ATCC Cat#TIB-152 MeWo ATCC Cat#HTB-65 A375 ATCC Cat#CRL-1619 SK-Mel-2 CRL-ITT, Pisa N/A SK-Mel-5 CRL-ITT, Pisa N/A SK-Mel-28 CRL-ITT, Pisa N/A M26C CRL-ITT, Florence N/A M33x CRL-ITT, Florence N/A U937 ATCC Cat#CRL-1593 Experimental Models: Organisms/Strains Athymic nude mice (Foxn1 nu/nu) Envigo N/A (Continued on next page) e2 Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms VegaZZ Drug Design Laboratory http://www.vegazz.net I-TASSER Zhang Lab http://zhanglab.ccmb.med.umich.edu/ I-TASSER Verify_3D UCLA- DOE LAB http://services.mbi.ucla.edu/Verify_3D/ Thermo ImageQuest ThermoFisher Cat#10137985 GraphPad Prism version 7.00 for Windows GraphPad Software https://www.graphpad.com/

    Lactate Assay:

    Article Title: Identification of the Nicotinamide Salvage Pathway as a New Toxification Route for Antimetabolites.
    Article Snippet: Cat#354234 NAD Sigma-Aldrich Cat#N3014 NADH Sigma-Aldrich Cat#N8129 ATP Sigma-Aldrich Cat#A2383 GLUCOSE [U-14C] Perkin Elmer Cat#NEC042V250UC TMRE Sigma-Aldrich Cat#87917 FCCP Sigma-Aldrich Cat#C2920 Nicotinamide Mononucleotide (NMN) Sigma-Aldrich Cat#N3501 Nicotinamide Riboside (NR) ChromaDex Cat#ASB-00014314 Nicotinamide (NAM) Sigma-Aldrich Cat#72340 5-Phospho-D-ribose 1-diphosphate (PRPP) Sigma-Aldrich Cat#P8296 Inosine 5’-monophosphate Sigma-Aldrich Cat#57510 DL-Glyceraldehyde 3’-phosphate Sigma-Aldrich Cat#G5251 Trizol Reagent ThermoFisher Cat#15596026 iScript BioRad Cat#1708890 SYBR Green PCR kit BioRad Cat#1725271 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat#A7906 Bovine Serum Albumin (BSA for cycling assay) Sigma-Aldrich Cat#A8531-1VL Riboflavin 50-monophosphate (FMN) Sigma-Aldrich Cat#F8399 Resazurin Sigma-Aldrich Cat#R7017 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma-Aldrich Cat#I6758 Tris(2-carboxyethyl)phosphine HCl (TCEP) Sigma-Aldrich Cat#75259 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy1-propanesulfonate (CHAPSO) Sigma-Aldrich Cat#C3649 DL-Dithiothreitol Sigma-Aldrich Cat#43819 Phenylmethanesulfonyl fluoride (PMSF) Fluka Cat#78830 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8849 Ampicillin Sigma-Aldrich Cat#A9518 Kanamycin Sigma-Aldrich Cat#K4000 HisTrap HP column GE Healthcare Cat#17-5247-01 PD-10 desalting columns (Sephadex G-25) GE Healthcare Cat#17-0851-01 PD MiniTrap (Sephadex )G-25 GE Healthcare Cat#28-9180-07 Supelcosil LC-18-S HPLC column Supelco (Sigma-Aldrich) Cat#58928-U Supelcosil LC-18-T HPLC column Supelco (Sigma-Aldrich) Cat#58971 (Continued on next page) Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Kinetex 2.6 mm C18 100A Phenomenex Cat#00F-4462-E0 Kinetex 2.6 mm HILIC 100A Phenomenex Cat#00F-4461-E0 TRACER EXTRASIL ODS2 5 mm Phenomenex Cat#N34544 Glyceraldehyde-3-phosphate Dehydrogenase Sigma-Aldrich Cat#G9263 Inosine Monophosphate Dehydrogenase Type II human Sigma-Aldrich Cat#I1782 Diaphorase from Clostridium kluyveri Sigma-Aldrich Cat#D5540 pET15b vector Novagen, EMD Millipore Cat#69661-3 Recombinant Human NAMPT This study N/A pTrcHisA- NMNAT1 Sorci et al., 2007 N/A pET28c- NMNAT2 Brunetti et al., 2010 N/A pTrcHisA- NMNAT3 Sorci et al., 2007 N/A Recombinant E.coli NMN deamidase (PncC) Zamporlini et al., 2014 N/A Recombinant B. anthracis NaMN adenylyltransferase (NadD) Zamporlini et al., 2014 N/A Recombinant B. anthracis NAD synthetase (NadE) Zamporlini et al., 2014 N/A a-cyano-hydroxycynnamic acid Sigma-Aldrich Cat#C2020 Aniline Sigma-Aldrich Cat#51788 9-aminoacridine Sigma-Aldrich Cat#92817 Oligonucleotides Oligonucleotides used, see Table S1 This study N/A Bacterial strains One Shot BL21 Star (DE3) Chemically Competent E.coli cells Invitrogen Cat#440049 One Shot TOP 10 Chemically Competent E.coli cells Invitrogen Cat#404003 Critical Commercial Assays ATPlite Luminescence Assay Perkin Elmer Cat#6016943 Bradford Protein Assay BioRad Cat#5000201 Lactate assay kit BioVision Cat#K607 Human NMNAT2 This study UniProtKB/Swiss-Prot: Q9BZQ4.1 Experimental Models: Cell Lines SH-SY5Y ATCC Cat#CRL-2266 HeLa ATCC Cat#CCL-2 INS-1 ATCC Cat#CRL-2057 SK-Hep-1 ATCC CatHTB-52 C6 ATCC Cat#CCL-107 NCI-H295R ATCC Cat#CRL-2128 CAPAN-1 ATCC Cat#HTB-79 JURKAT ATCC Cat#TIB-152 MeWo ATCC Cat#HTB-65 A375 ATCC Cat#CRL-1619 SK-Mel-2 CRL-ITT, Pisa N/A SK-Mel-5 CRL-ITT, Pisa N/A SK-Mel-28 CRL-ITT, Pisa N/A M26C CRL-ITT, Florence N/A M33x CRL-ITT, Florence N/A U937 ATCC Cat#CRL-1593 Experimental Models: Organisms/Strains Athymic nude mice (Foxn1 nu/nu) Envigo N/A (Continued on next page) e2 Cell Chemical Biology 25, 1–12.e1–e7, April 19, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms VegaZZ Drug Design Laboratory http://www.vegazz.net I-TASSER Zhang Lab http://zhanglab.ccmb.med.umich.edu/ I-TASSER Verify_3D UCLA- DOE LAB http://services.mbi.ucla.edu/Verify_3D/ Thermo ImageQuest ThermoFisher Cat#10137985 GraphPad Prism version 7.00 for Windows GraphPad Software https://www.graphpad.com/



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    Figure 2. Schematic representation of circular miR- 21 decoys generation using a tRNA-splicing mechanism and LM21D The secondary structure of the Drosophila Tyr-tRNA- CM21D, CM21D, CM21SD, and LM21D was predicted by MXfold2 and visualized using VARNA v3.93 <t>(RRID:</t> SCR_006459). tRNA (dark green), miR-21 bulged binding site (purple), miR-21 perfect binding site (light purple), miR-21 scrambled binding sites (orange), and RNA aptamer (light green). Red arrows indicate splice sites in Tyr-tRNA CM21D. The NotI (yellow) and SacII (light blue) restriction sites can be used to replace CM21D with CM21SD. Yellow arrows indicated the junction sites on CM21D and CM21SD.
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    Figure 2. Schematic representation of circular miR- 21 decoys generation using a tRNA-splicing mechanism and LM21D The secondary structure of the Drosophila Tyr-tRNA- CM21D, CM21D, CM21SD, and LM21D was predicted by MXfold2 and visualized using VARNA v3.93 <t>(RRID:</t> SCR_006459). tRNA (dark green), miR-21 bulged binding site (purple), miR-21 perfect binding site (light purple), miR-21 scrambled binding sites (orange), and RNA aptamer (light green). Red arrows indicate splice sites in Tyr-tRNA CM21D. The NotI (yellow) and SacII (light blue) restriction sites can be used to replace CM21D with CM21SD. Yellow arrows indicated the junction sites on CM21D and CM21SD.
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    Figure 2. Schematic representation of circular miR- 21 decoys generation using a tRNA-splicing mechanism and LM21D The secondary structure of the Drosophila Tyr-tRNA- CM21D, CM21D, CM21SD, and LM21D was predicted by MXfold2 and visualized using VARNA v3.93 <t>(RRID:</t> SCR_006459). tRNA (dark green), miR-21 bulged binding site (purple), miR-21 perfect binding site (light purple), miR-21 scrambled binding sites (orange), and RNA aptamer (light green). Red arrows indicate splice sites in Tyr-tRNA CM21D. The NotI (yellow) and SacII (light blue) restriction sites can be used to replace CM21D with CM21SD. Yellow arrows indicated the junction sites on CM21D and CM21SD.
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    Figure 2. Schematic representation of circular miR- 21 decoys generation using a tRNA-splicing mechanism and LM21D The secondary structure of the Drosophila Tyr-tRNA- CM21D, CM21D, CM21SD, and LM21D was predicted by MXfold2 and visualized using VARNA v3.93 <t>(RRID:</t> SCR_006459). tRNA (dark green), miR-21 bulged binding site (purple), miR-21 perfect binding site (light purple), miR-21 scrambled binding sites (orange), and RNA aptamer (light green). Red arrows indicate splice sites in Tyr-tRNA CM21D. The NotI (yellow) and SacII (light blue) restriction sites can be used to replace CM21D with CM21SD. Yellow arrows indicated the junction sites on CM21D and CM21SD.
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    Figure 2. Schematic representation of circular miR- 21 decoys generation using a tRNA-splicing mechanism and LM21D The secondary structure of the Drosophila Tyr-tRNA- CM21D, CM21D, CM21SD, and LM21D was predicted by MXfold2 and visualized using VARNA v3.93 (RRID: SCR_006459). tRNA (dark green), miR-21 bulged binding site (purple), miR-21 perfect binding site (light purple), miR-21 scrambled binding sites (orange), and RNA aptamer (light green). Red arrows indicate splice sites in Tyr-tRNA CM21D. The NotI (yellow) and SacII (light blue) restriction sites can be used to replace CM21D with CM21SD. Yellow arrows indicated the junction sites on CM21D and CM21SD.

    Journal: Molecular therapy. Nucleic acids

    Article Title: Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastoma in vitro and in vivo models.

    doi: 10.1016/j.omtn.2023.04.001

    Figure Lengend Snippet: Figure 2. Schematic representation of circular miR- 21 decoys generation using a tRNA-splicing mechanism and LM21D The secondary structure of the Drosophila Tyr-tRNA- CM21D, CM21D, CM21SD, and LM21D was predicted by MXfold2 and visualized using VARNA v3.93 (RRID: SCR_006459). tRNA (dark green), miR-21 bulged binding site (purple), miR-21 perfect binding site (light purple), miR-21 scrambled binding sites (orange), and RNA aptamer (light green). Red arrows indicate splice sites in Tyr-tRNA CM21D. The NotI (yellow) and SacII (light blue) restriction sites can be used to replace CM21D with CM21SD. Yellow arrows indicated the junction sites on CM21D and CM21SD.

    Article Snippet: Cell culture, transfection, and generating stable cell line Human HEK293T (ATCC Cat# CRL-3216, RRID: CVCL_0063) and GBM cell lines, 1321N1 (Sigma-Aldrich Cat# 86030402, RRID: CVCL_0110), A172 (ATCC Cat# CRL-1620, RRID: CVCL_0131), U87 (ATCC Cat# HTB-14, RRID: CVCL_0022), and rat GBM cell line, C6 (ATCC Cat# CCL-107, RRID: CVCL_0194), were cultured in DMEM-F12 (Gibco, Catalog: 12500096) supplemented with 10% FBS (GIBCO, Catalog: A4736401) and 1% penicillin-streptomycin (GIBCO, Catalog:10378016) under optimal growth conditions.

    Techniques: Binding Assay